ABSTRACT
Objective To compare the feasibility and safety of laparoscopic partial renal segmental artery occlusion and renal artery trunk interruption for partial nephrectomy. Methods We reviewed medical records of 65 patients with stage T1 renal tumor from October 2013 to February 2017. Among them, 29 cases underwent partial nephrectomy with high selective segmental renal artery occlusion, and 36 cases underwent partial nephrectomy with renal artery trunk occlusion. Then compare the preoperative and postoperative creatinine changes, intraoperative blood loss, warm ischemia time, operation time, hospitalization time and positive margins of the two methods. Results In all the patients, the procedures were done without conversion to open surgeries. The operation time of the renal artery branch block group is shorter than that of the main renal artery block, but the preoperative and postoperative creatinine changes are smaller than those of the main renal artery occlusion group. There was no significant difference between the two groups in intraoperative blood loss, warm ischemia time and positive margins (P > 0.05); There was no significant difference between the two groups in age, body mass index (BMI) and R.E.N.A.L score of renal tumor (P > 0.05). Conclusion For the stage T1 renal tumor, the selective renal artery branch interruption technique has a longer operation time, but the renal tissue has a small ischemic zone and has little effect on the kidney.
ABSTRACT
Objective:To investigate the effect of miR-34a on the migration and invasion of prostatic cell line through MSR1.Methods:Western blot was used to detect the expression of MSR1 in prostate cancer cell lines.qPCR was used to detect the ex-pression of miR-34a in prostate cancer cell lines.The correlation between miR-34a and MSR1 was examined by double luciferase as-say.Transwell invasion assay was used to detect the effect of miR-34a and MSR1 on the invasion ability of prostate cancer cells.The effects of miR-34a and MSR1 on the fine migration ability of prostate cancer were examined by scratch healing.Results:The expression level of MSR1 in PC3 cells was relatively high by Western blot.The expression level of miR-34a in PC3 cells was relatively low by qPCR.Double luciferase assay showed a direct binding site between miR-34a and MSR1,MiR-34a could regulate the expression level of MSR1,Scaffold Healing Test and Transwell Invasion Test were used to detect the migration and invasion of PC3 cells after overexpressing miR-34a.Overexpression of MSR1 could reverse the inhibitory effect of miR-34a on the migration and invasion of PC3 cells.Conclusion:miR-34a can regulate the migration and invasion of prostate cancer cells through MSR1 protein.
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of the nucleostemin (NS) gene in prostate cancer PC-3, LNCaP and DU145 cells, and to study the effect of the NS gene on the proliferation of PC-3 cells after its silencing.</p><p><b>METHODS</b>The protein and mRNA expressions of NS in PC-3, LNCaP and DU145 cells were respectively detected by immunohistochemical staining and RT-PCR. An NS-specific short-hairpin RNA (shRNA) expression plasmid was used to transfect the PC-3 cells (NS-shRNA-PC-3), followed by observation of the changes of the NS gene and the proliferation and apoptosis of the cells.</p><p><b>RESULTS</b>The NS gene was highly expressed in the three types of cells. After the transfection, the NS expression and the proliferation of the NS-siRNA-PC-3 cells were remarkably reduced, while the percentage of the GO/G1 cells and the early apoptosis of the PC-3 cells obviously increased. A marked decrease was observed in the neoplasm forming ability of the NS-siRNA-PC-3 cells in the nude mice.</p><p><b>CONCLUSION</b>NS is highly expressed in prostate cancer cells. The proliferation of PC-3 cells is remarkably reduced and the early apoptosis of PC-3 cells increased after silencing the NS gene by NS-specific shRNA.</p>
Subject(s)
Animals , Humans , Male , Mice , Apoptosis , Carrier Proteins , Genetics , Cell Line, Tumor , Cell Proliferation , GTP-Binding Proteins , Gene Silencing , Mice, Nude , Neoplasm Proteins , Genetics , Nuclear Proteins , Genetics , Prostatic Neoplasms , RNA, Small Interfering , Reverse Transcriptase Polymerase Chain Reaction , TransfectionABSTRACT
<p><b>OBJECTIVE</b>To screen the genes and possible signal transduction pathways involved in the mechanism of nucleostemin (NS) in the proliferation of prostate cancer.</p><p><b>METHODS</b>Oligonucleotide DNA microarray was used to screen the genome changes after knocking-down expression of NS in PC-3 cells and quantitative real-time PCR was used to further confirm the important differentially expressed genes.</p><p><b>RESULTS</b>219 differentially expressed genes were found and theses genes were involved in cell cycle, cell proliferation, signal transduction, cell apoptosis and cell differentiation, etc. INK4 family genes (p15, p16, p18) were up-regulated and cyclin D1, HDAC1 were down-regulated, the main action points were CDK4/6-cyclin D and pRb-E2F1 complexes.</p><p><b>CONCLUSION</b>NS may promote the progression of prostate cancer by inhibiting the expression of p15, p16, and p18 in PC-3 cells. NS is an important G(1)/S checkpoint regulator and its regulatory activity has been certified at gene level.</p>
Subject(s)
Humans , Male , Apoptosis , Cell Cycle , Cell Line, Tumor , Cell Proliferation , Cyclin D1 , Metabolism , Cyclin-Dependent Kinase Inhibitor Proteins , Metabolism , GTP-Binding Proteins , Genetics , Gene Expression Profiling , Gene Expression Regulation, Neoplastic , Gene Knockdown Techniques , Histone Deacetylase 1 , Metabolism , Nuclear Proteins , Genetics , Oligonucleotide Array Sequence Analysis , Prostatic Neoplasms , Genetics , Metabolism , Pathology , RNA Interference , Signal TransductionABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of the nucleostemin (NS) gene in prostate cancer (PCa) tissues and its clinical significance.</p><p><b>METHODS</b>We detected the NS expression in PCa, benign prostatic hyperplasia (BPH) and high grade prostatic intraepithelial neoplasia (HGPIN) tissues by RT-PCR and immunohistochemistry, and analyzed the correlation between the expression of the NS protein and the clinical variables of PCa.</p><p><b>RESULTS</b>The NS mRNA level was markedly higher in the PCa than in the BPH tissues. The rates of strongly positive, positive and weakly positive expressions of the NS protein were 48.8%, 36.6% and 12.2% in PCa, 4.0%, 32.0% and 56.0% in BPH, and 5.0%, 25.0% and 60.0% in HGPIN, respectively. The expression level of the NS protein was significantly higher in PCa than in BPH and HGPIN (P < 0.05). The expression of the NS gene was negatively correlated with the degree of cell differentiation in the PCa tissues, the worse the differentiation, the higher the NS expression level.</p><p><b>CONCLUSION</b>The NS gene is highly expressed in PCa tissues and may have an important role in the adverse differentiation and malignant proliferation of prostate cancer.</p>